Label-Free Microfluidic Characterization of Temperature-Dependent Biomolecular Interactions
News Oct 13, 2011
Solvated L-arginine vasopressin (AVP) and its immobilized RNA aptamer (spiegelmer) were allowed to achieve equilibrium binding in a microchip at a series of selected temperatures. Unbound AVP were collected and analyzed with matrix-assisted laser desorption∕ionization mass spectrometry (MALDI-MS), yielding melting curves that reveal highly temperature-dependent zones in which affinity binding (36-45 °C) or dissociation (25-33 °C and 50-65 °C) occurs. Additionally, temperature-dependent binding isotherms were constructed; from these, thermodynamic quantities involved in binding were extracted. The results illustrated a strong change in heat capacity of interaction for this system, suggesting a considerable thermodynamic influence controlling vasopressin-spiegelmer interaction.
The article is published online in Biomicrofluidics and is free to access.
Diagnosing Immunity to Ebola With Paper Tests & SmartphonesNews
A promising new approach to detect immunity to Ebola virus infection has been developed by researchers from i-sense in a collaboration between UCL and Imperial College London.READ MORE
New Technique Eases Production, Customization of Soft RoboticsNews
By helping rubber and plastic stick together under pressure, University of Nebraska-Lincoln chemists have simplified the production of small fluid-carrying channels that can drive movement in soft robotics and enable chemical analyses on microscopic scales.READ MORE
New Preservation Method Maintains Viable CTCs for up to 72 HoursNews
A new blood stabilization method significantly prolongs the lifespan of blood samples for microfluidic sorting and transcriptome profiling of rare circulating tumor cells.READ MORE