Transplanted human stem cells treated with drug boost myelin synthesis and improve auditory function in a preclinical study
Damage to myelin, the fatty insulator that enables communication between nerve cells, characterizes multiple sclerosis (MS) and other devastating neurological diseases.
The damage doesn’t come all at once: There is a “honeymoon” period during which some regeneration of myelin, called remyelination, does occur, but this ability to regenerate dissipates as the disease progresses and the patient ages.
Now, a University at Buffalo researcher has discovered a way to keep that kind of remyelination going, using a drug that’s already on the market.
A paper describing the research results was published in the Journal of Neuroscience.
“We have identified a new drug target that promotes stem cell therapy for myelin-based disease, such as MS,” says lead author Fraser J. Sim, PhD, assistant professor in the Department of Pharmacology and Toxicology in the University at Buffalo School of Medicine and Biomedical Sciences.
The study shows it is possible to boost myelination by targeting human oligodendrocyte progenitor cells with solifenacin, an anti-muscarinic drug that currently is approved and marketed to treat overactive bladder.
“Our hypothesis is that in MS, the oligodendrocyte progenitor cells seem to get stuck,” Sim explains. “When these cells don’t mature properly, they don’t differentiate into myelinating oligodendrocytes.”
In the new study, the approach Sim and his colleagues took was to first characterize the molecular pathways that govern the differentiation of human oligodendrocyte progenitor cells, and then identify drug candidates that would promote differentiation and myelin production.
They found that when a muscarinic type 3 receptor on human oligodendrocyte progenitor cells was activated, differentiation was completely blocked.
“So we thought, if we had something that blocks instead of activates this receptor, could we boost differentiation?” asks Fraser. To do that, the researchers turned to solifenacin, the anti-muscarinic drug for overactive bladder; the bladder muscle contains several muscarinic receptors.
“We were excited about this because solifenacin is an approved drug that’s already on the market,” says Sim.
To test whether the drug could boost myelin synthesis, the researchers transplanted human oligodendrocyte progenitor cells into mice that could not make myelin. The result was increased differentiation and myelin synthesis from the transplanted human cells.
But Sim and his colleagues needed a functional endpoint, a way to know that the myelin being made was being translated into improved behavior or function.
So Sim teamed up with Richard J. Salvi, PhD, SUNY Distinguished Professor in the Department of Communicative Disorders and Sciences, and director of UB’s Center for Hearing and Deafness.
Together, they determined that an auditory brainstem response, which records brain wave activity in response to sounds, would be appropriate.
Sim explains that it takes a certain amount of time for a signal to go from the ear to the front of the brain: “So in the readout, you get waves that should have a certain time pattern. When there isn’t enough myelin, the signaling slows down. And if you add myelin, you should see the signals speed up.”
The tests showed improvement in the response to auditory signals in animals transplanted with the human oligodendrocyte progenitor cells treated with solifenacin.
“We have identified a way to improve human myelination,” says Sim.
The promising results have prompted Sim and his colleagues to seek funding for a small human trial.
The study results are all preclinical and no human testing has been done yet.
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Kavitha Abiraman, Suyog U. Pol, Melanie A. O'Bara, Guang-Di Chen, Zainab M. Khaku, Jing Wang, David Thorn, Bansi H. Vedia, Ezinne C. Ekwegbalu, Jun-Xu Li, Richard J. Salvi, Fraser J. Sim. Anti-Muscarinic Adjunct Therapy Accelerates Functional Human Oligodendrocyte Repair. Journal of Neuroscience, Published February 25 2015. doi: 10.1523/JNEUROSCI.3510-14.2015