NuGEN Technologies, Inc. and Kreatech Biotechnology have announced an agreement that will enable NuGEN to market FL-Ovation™ cDNA Fluorescent Modules designed to complement its family of Ovation™ RNA amplification products.
Together, the systems will provide life science researchers with a flexible and sensitive RNA sample preparation workflow for use on Agilent DNA microarrays for gene expression analysis.
The non-exclusive agreement calls for Kreatech to supply components for a NuGEN-validated fragmentation and labeling system for one- and two-color microarray analysis utilizing its patented Universal Linkage System (ULS™) technology for labeling with Cy™3 and Cy™5 mono NHS esters licensed by NuGEN from GE Healthcare.
The NuGEN FL-Ovation™ cDNA Fluorescent Modules, along with the NuGEN Ovation™ RNA amplification family of products will help researchers unlock discoveries hidden within a broad range of samples including FFPE, LCM, blood, saliva, tissue biopsies, and others.
Using as little as picograms of total RNA, NuGEN Ovation Systems allow small, degraded, and challenging RNA samples to be amplified into micrograms of cDNA. The amplified cDNA can be fragmented and labeled for same-day hybridization to Agilent DNA microarrays or used for direct qPCR analysis.
The new module now makes the Ovation family of products available to researchers who use the Agilent platform, enabling detection of extremely low-abundance transcripts, and raising the level of sensitivity for global gene expression analysis.
“With technology from Kreatech and GE Healthcare, NuGEN can now deliver the Ovation RNA amplification system to researchers conducting gene expression analysis on the Agilent platform,” explained Sue Pandey, Ph.D., vice president of commercial operations for NuGEN Technologies.
“Our customers have asked us to deliver an Ovation solution enabling whole genome expression analysis for the Agilent platform—and we’ve created a simple, easy-to-use, yet highly reproducible and sensitive solution based on our proven Ovation technology.”