We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
TTP Labtech, UC San Diego Collaborate
News

TTP Labtech, UC San Diego Collaborate

TTP Labtech, UC San Diego Collaborate
News

TTP Labtech, UC San Diego Collaborate

Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "TTP Labtech, UC San Diego Collaborate"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

TTP Labtech Ltd and the University of California San Diego (UC San Diego) have announced that their collaboration has resulted in a new workflow for single cell sequencing, which reduces the cost of sample preparation by 90%. The workflow, which has been published in the Journal of Laboratory Automation, was developed through the combination of UC San Diego’s expertise in genomics and the use of TTP Labtech’s mosquito liquid handlers, which can pipette nanolitre to microlitre volumes with high accuracy and reproducibility, to miniaturise and automate the process.

Although single-cell genomics is advancing rapidly, researchers face challenges linked with reproducibility, sensitivity, scalability and cost, particularly when trying to miniaturise high-throughput applications. To maximise accuracy and precision, library prep protocols recommend volumes that are within the range of manual pipettes or large-volume liquid handlers. However, only a small proportion of each of the libraries prepared will be required for sequencing. The focus of UC San Diego’s partnership with TTP Labtech was to miniaturise single-cell RNA library prep reaction set-up, to minimise sample and reagent requirements.

The resulting workflow has been shown to reduce library prep costs by up to 90% while maintaining throughput, accuracy and reproducibility. The miniaturisation of library prep volumes was achieved using TTP Labtech’s mosquito HTS and HV liquid handlers, which accurately dispense volumes between 25 nL and 1.2 µL and 0.5 to 5 µL, respectively. Both instruments use true positive-displacement pipetting technology, which maintains volumetric accuracy even for highly viscous liquids, enabling high concentration gDNA to be handled, to save on reagent cost and sample input.

To assess the quality of the libraries, UC San Diego applied the workflow to analyse pancreatic differentiation of human embryonic stem cells. The resulting single-cell RNA-sequencing data were analysed to determine the reproducibility of this system and its ability to distinguish not only between cells at different stages of differentiation but also between individual cells within each stage.

Joby Jenkins, Product Manager, TTP Labtech, commented: “Single cell sequencing requires large numbers of samples to be handled in order to provide valid data based on population studies. Reproducibility and sensitivity, as well as scalability and cost, can be limiting factors when large numbers of cells are analysed, and we are very pleased that our partnership with UCSD has achieved our goal of improving sample prep for single cell genomics research.”

Dr Louise Laurent, MD, PhD, Associate Professor, UC San Diego, said: “The workflow we have developed, in partnership with TTP Labtech, has the potential to provide researchers with significant cost savings while maintaining the quality of single cell sequencing. The instrument cost and their ease-of-use make the mosquito liquid handlers viable options for labs that would like to scale-down and automate next-generation sequencing sample preparation.” TTP Labtech will be presenting a poster on the published workflow at the Single Cell Genomics Conference, 14-16 September 2016, Wellcome Genome Campus, Hinxton, UK and exhibiting at ASHG 2016 booth #1326, 18-22 October 2016, Vancouver, BC, Canada.

Advertisement