Identification and Characterisation of Disease Resistance Pathway Genes in Jute (Corchorus spp.)
Poster Mar 05, 2012
Niaz Mahmood, Salma Sultana Alam, Rifit Ara Najnin, Nurun Nahar Fancy, Haseena Khan.
Plant disease resistance genes, mainly known as the R genes, play a vital role in plant defence system through encoding proteins for pathogen detection. Most plant R genes have a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) region. The NBS region is preceded either by a coiled-coil (CC) domain or a TIR (Toll/interleukin-1 receptor like) domain. With a view to identifying and characterizing all LBS domain containing genes in jute, degenerate primers were designed from the conserved MBS domain. Subsequent cloning and sequencing identified the presence of both CC-NBS-LRR and TIR-NBS-LRR domain containing disease resistance genes.
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation SystemPoster
Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays.READ MORE
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Optimized Workflow for Single Cell Copy Number Profiling Using High Resolution Oligo CGH ArraysPoster
Here we describe GenetiSure Pre-Screen, a same day, cost-effective, analysis workflow that combines whole genome amplification (WGA) with copy number (CN) profiling using high-resolution oligo CGH microarrays.READ MORE