Expanding Practical Education With Brightfield Imaging-Enabled Flow Cytometry
Insights and Expanding Practical Education in a Core Facility With Brightfield Imaging-Enabled Flow Cytometry
Maximize mRNA Yield Using In Vitro Transcription
The growing use of RNA-based vaccines and therapies has focused much attention and effort on developing conditions for maximizing the yield and efficiency of the in vitro transcription (IVT) reactions used to produce mRNA. IVT procedures involve the biosynthesis of RNA from a linear DNA template containing an appropriate transcription promoter. But how do different buffer conditions and nucleotide concentrations impact the IVT reaction?
Lead Identification Screening Services in 3D
2D cultures often fall short in drug screening when it comes to clinical predictivity, but 3D models and high content services allow accurate and clinically relevant screens.
Optimized Expression of Membrane Proteins: New Tools for Difficult-To-Express Proteins
Membrane proteins are a notoriously difficult class of proteins to express, purify and characterize. Expression levels of membrane proteins in mammalian expression systems, the preferred system to generate native-like post-translational modifications, are often so low that yields are unsuitable for the intended downstream uses.
Fluorophore and Reagent Selection Guide for Flow Cytometry
Researchers are always developing innovative applications for novel research areas, improving data quality and expanding the boundaries of experimental limitations.
Tackle Real-Time PCR Research Challenges
Nucleic acid amplification and detection techniques are highly valuable tools that can be used across a wide range of applications... Real-time PCR, also known as quantitative PCR (qPCR), is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets.
Selecting the Right HPLC Column
C18 columns have a broad applicability, from pharmaceuticals to food and environmental analyses. However versatile C18 columns are, they aren't the optimal stationary phase for every application.
What Are the Compounds Released from Coffee Filter Paper?
Most techniques utilize a filter to hold the ground coffee during the brewing process. Coffee filters can reduce some of the acidity and oils from the coffee, but if they are not rinsed, negative aromas can be extracted during the brewing process. This can lead to a cup of coffee with paper, plastic and other off-odor aromas.
Pesticide Residue Analysis With Automated EDGE Extraction System and Multi-Layer GC-MS Sandwich Injection
The QuEChERS method is the industry standard for pesticide clean-up and extraction of a wide variety of food samples. QuEChERS kits are easy to use and enable fast sample preparation resulting in excellent pesticide recoveries.
Enhancing Histological Tissue and Cell Characterization With Simultaneous Gene Expression and Protein Measurements
Cells establish their function, identity and state through the careful orchestration of complex molecular mechanisms leading to gene expression. To characterize cells more accurately, it is important to evaluate gene expression at both transcript and protein levels.