Latest Posters
Poster
Multifunctional, “Smart”, Polymeric Microfluidics Fabricated by Plasma Processing: Applications in Capillary Filling, and Passive Superhydrophobic Valving
We demonstrate a mass-production amenable technology for fabrication, surface modification and multifunction integration in plastic, disposable microfluidic devices, namely direct lithography on the plastic substrate followed by polymer plasma etching, and if desired by selective plasma deposition. We apply the plasma processing technology to fabricate polymeric microfluidics in Poly(methyl methacrylate) (PMMA) and Poly(ether ether ketone) (PEEK). Our approach proposes “smart” multifunctional mi
Poster
Integration of predictive model with microfluidics fabrication using a 193 nm excimer laser source shaped by an Intelligent Pinhole mask
Simulation, analysis and fabrication of microfluidic geometries using a laser-based fabrication process incorporating a reconfigurable mask. A LabVIEW development programme provides geometrical analysis prior to fabrication. The same programme is then used to control the mask during the machining step.
Poster
Proteomic non-small cell lung carcinoma biomarker screening in bronchoalveolar lavage fluid
Using a standardized method to acquire MALDI-TOF proteome profile spectra of bronchoalveolar lavage fluid (BALF), we have shown the upregulation of histatin 3 and calgranulin C in a small pilot cohort of NSCLC patients. This pilot study serves to demonstrate that it is feasible to screen a larger NSCLC patient cohort for BALF proteome level biomarkers in a clinical setting.
Poster
A Novel Approach to Internal Standardization in LC/MS/MS Analysis; Sensitive LC/MS/MS Analysis of Gentamicin
A novel approach to internal standardization in LC/MS/MS is demonstrated. Gentamicin, a multi-component aminoglycoside, is derivatized with propyl-d7 chloroformate making the internal standard. Gentamicin components in the samples are derivatized with non-labeled propyl chloroformate making them analogous to the internal standard. Additional benefits of the derivatization are increased hydrophobicity and excellent MS/MS fragmentation.
Poster
FOXP3 Gene Expression in Multiple Sclerosis patients before and after Mesenchymal Stem Cell therapy
Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disorder of the CNS. No successful treatment for MS, but one therapeutic strategy in research is the use of bone marrow-derived mesenchymal stem cells (MSC). We studied a group of MS patients who underwented MSCs, assayed for expression of a transcription factor, FOXP3, as a specific marker of MS amelioration in peripheral blood. qRT-PCR on PBMCs showed higher FoxP3 levels.
Poster
Fragment Screening Using Capillary Electrophoresis
Capillary electrophoresis, which was pioneered for HTS in the 1990s, has recently been adapted to enable the screening of fragments. The technique is explained and the screening of fragments against a chaperone protein Hsp90 is presented as an example.
Poster
High-Throughput Multiplexed Inflammation Assay Using Primary Cells
An important event in the inflammation response is the expression of cell-surface antigens that facilitate binding of immune cells to blood vessels. The ability to monitor up-regulation of these molecules, such as VCAM, E-selectin, and HLADR on endothelial cells provides an important physiological read-out for cell-based models of inflammation.
Poster
Maximizing cell wash performance with the AquaMax® 2000/4000 microplate washers
Many cell-based applications, including calcium flux assays, cell-based ELISAs, ion channel assays, and others require gentle but thorough washing of cells in order to ensure cell retention, cell integrity and optimal assay performance. The 96 cell wash head for AquaMax® 2000/4000 microplate washers from Molecular Devices uses angled pins to dispense fluid without disturbing cells. Adjustable dispense rate and aspiration height allow users to minimize disruption of even weakly adherent cells. We
Poster
Two secreted orthogonal bioluminescent reporters for non-destructive ultrasensitive assays
Here we present a powerful combination of two secreted bioluminescent reporters, the luciferases from Gaussia princeps and Cypridina noctiluca. We demonstrate that the properties of these two reporters in terms of sensitivity, compatibility and use in non- destructive assays conducted on live cells or supernatants from different well formats makes them suitable for novel high throughput format applications.
Poster
High-Throughput Multiplexed Assay for Analysis of Hematopoietic Stem Cells Differentiation and Hematopoietic Toxicity
Hematopoietic stem cells (HSCs) give rise to all the blood cell types and are important for cell therapy and drug development. During the development of lymphoid and myeloid lineages, HSC differentiate into committed hematopoietic progenitors. Monitoring the expansion and differentiation of HSCs into lineage-commited hematopoietic progenitors is important for research of hematopoiesis and developing therspeutic processes with HSCs
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