Latest Posters
Poster
High-Throughput Multiplexed Assay for Analysis of Hematopoietic Stem Cells Differentiation and Hematopoietic Toxicity
Hematopoietic stem cells (HSCs) give rise to all the blood cell types and are important for cell therapy and drug development. During the development of lymphoid and myeloid lineages, HSC differentiate into committed hematopoietic progenitors. Monitoring the expansion and differentiation of HSCs into lineage-commited hematopoietic progenitors is important for research of hematopoiesis and developing therspeutic processes with HSCs
Poster
miR-21 upregulation and miR-128a downregulation in human glioblastomas
miRNA deregulation is an important hallmark on cancer development and progression. This work shows that miR-21 is overexpressed is human glioblastoma samples and cell lines, whereas miR-128a is highly downregulated on these tumours. miRNA modulation using antisense oligonucleotides and/or miRNA precursor molecules decreased tumor cell viability. Thus, targeting deregulated miRNAs may constitute a complementary/alternative approach on the treatment of glioblastomas and other miRNA-related maligna
Poster
Label free technology: the weird and wonderful world of cell signaling
Whilst conducting an evaluation of label free technology platforms we
encountered a number of surprising, but reproducible, observations. For
example, receptor over-expression impacts endogenous signaling
responses; surface coatings affecting cell adherence can change the
response profile; and it is important to choose the appropriate analysis
metric for complex profiles. Awareness of the subtleties of cell signaling
within model systems such as CHO and HEK recombinant over-expression
sys
Poster
Label free technology: value added information in the drug discovery process
We have evaluated Molecular Devices CellKey™ and SRU BIND® label free
detection technologies and their application to hit finding and hit validation
using cell based assays. The presented data highlights:
Poster
Receptor Interacting Protein 140 (RIP140) silencing via RNAi interferes with fatty acid metabolism but not with glucose uptake in L6 muscle cells
The role of RIP140 in the regulation of fatty acid uptake, oxidation and glucose uptake was investigated in muscle cells using RNAi technique. Cells were incubated ±insulin before measurement of glucose and FA kinetics. We suggest that reducing RIP140 expression in muscle has no impact on the regulation of GU but leads to high rates of FAU and reduced sensitivity to insulin as it pertains to FA metabolism.
Poster
Co-rgulation of tumour suppressor gene programmed cell death 4 (PDCD4) by miR-21 and miR-499 in head and neck cancer
This study has identified differentially expressed miRNAs in tonsil cancers and provides new evidence for co-regulation of the oncogene PDCD4 by miR021 and miR-499
Poster
Hyaluronan-Chitosan Polyelectrolytic Complex as a Platform for Delivery of Cytotoxic Agents
Described here is a biomimetic hydrogel adapted to serve as a high concentration, regional delivery system for cytotoxic agents. Paclitaxel was conjugated with low molecular weight hyaluronan to render it water soluble, then embedded in the HCP-h hydrogel for delivey to a culture of C8161 malignant melanoma cells. Live/dead staining of cells was used to monitor effectiveness of paclitaxel release from its HCP-h depot.
Poster
Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the POLARstar Omega.
The Oris™ Cell Migration Assays from Platypus offer certain advantages over traditional migration assays. Especially the TriCoated kits enable the researcher to test the effect of different extracellular matrix components like Collagen I or Fibronectin on migration. In this application note we show the real-time monitoring of cell migration using a POLARstar Omega from BMG LABTECH equipped with a CO2 gas vented system.
Poster
The Transcreener® ADP2 Universal Kinase Assay from BellBrook Labs is readily performed on BMG LABTECH microplate readers using different assay formats
The Transcreener® ADP2 FI assay kit from BellBrook Labs is a simple one-step competitive red fluorescence immunoassay based on the detection of ADP. In this application note we show that this assay is compatible with four different microplate readers from BMG LABTECH. With the PHERAstar FS and Plus, as well as the POLARstar and FLUOstar Omegas comparable standard curves and EC50 values were obtained.
Poster
Enzyme kinetic measurements performed on BMG LABTECH´s FLUOstar OPTIMA
BMG LABTECH has developed a new evaluation software feature able to calculate the Km value as well as the maximal velocity (Vmax) from an enzymatic kinetic measurement.
Enzymes differ in their affinity to specific substrates and this affinity is represented by the Michaelis-Menten constant Km. The new software feature for enzyme kinetic offers fast and easy calculation of Km and Vmax. Available plots are the common Michaelis-Menten, Lineweaver-Burk, Eadie-Hofstee, Scatchard and Hanes kinetic f
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