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Microscope-based high-content instruments offer high optical resolution, however, the limited field of view afforded by their objective lenses can mean lengthy read times for some assays, especially where multiple image capture per well is required. Wherever possible, only a small percentage of the total number of cells present in a test well are analysed to keep plate read times at a minimum, which may not always be ideal. Microplate laser- scanning cytometers, such as TTP LabTech’s Acumen® eX3

The physical and chemical composition of biological samples is more diverse than the libraries of medicinal compounds used for drug discovery research. Types of samples include proteins, nucleic acids, blood and serum samples, cell suspensions, tissue biopsies, and antibodies. Thus any storage system must be flexible to accommodate the differing storage requirements of each sample type base upon its biochemical composition, stability and where appropriate, solvent.

mosquito® is a positive displacement liquid handling system capable of pipetting solutions with a high degree of accuracy (to within 5%) and precision (CVs of <10%) in the nanolitre range. Here, we demonstrate mosquito’s ability to dispense accurately low volumes of organic solvents of low surface tension and to re-dissolve compounds which have been previously dried in storage wells.

384-well plate “mini-tube” consumables have been developed by automation companies such as REMP and The Automation Partnership (TAP) to hold compound samples divided into single-use storage tubes. Each tube holds 40 -75 µL, and is filled, sealed, stored and then thawed just once before use. However, when accessed by conventional pipetting technology, the tube’s practical working volume is as little as 20 µL, which means high compound wastage. The seal must also be pierced prior to pipetting to p

A library of oxazolidin-2-ones was prepared by solid phase synthesis. Crutial reaction steps were optimized. Highly divers building blocks were selected for the synthesis. All compounds were purified using preparative LC/MS with mass directed fraction collection.

The formation of aromatic carbon-heteroatom bonds has traditionally been achieved by nucleophilic aromatic substitution or via the copper-mediated Ullman reaction. The palladium-catalyzed formation of aromatic C-N bonds extensively developed by Hartwig and Buchwald has provided a powerful alternative. While the aryl amination reaction is applicable even to aryl chlorides and activated phenols, the analogous C-O and C-S bond forming reactions have attracted less attention.

Central nervous system disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s, schizophrenia and anxiety, the success rate of CNS drug discovery programmes must be improved. The challenge in drug discovery is to develop more relevant assay systems to identify lead compounds for further development. Cell based screening assays currently used for CNS drug development involve primary cells or commercially

The authors present a result of survey among 300 residents of the Russian Far East on their potential willingness to store cord blood stem cells of their newborns and/or their own gametes for the future needs. The current status of the cord blood banking system in Russia is also described.

Human class Ia PI3 kinase p110alpha catalytic and p85alpha regulatory subunits were expressed and purified. A luminometric ATP depletion assay to measure PI3K activity was established and optimized. Furthermore, the autophosphorylation status of PI3K alpha was checked and the IC50 value for the known PI3K inhibitor wortmannin was determined. The assay is homogenous and is potentially suitable for high throughput screening to identify PI3K inhibitors.

The aim of this study is to produce a vector capable of expressing green fluorescent protein (GFP) when exposed to a CYP1A1 inducer.