Latest Posters
Poster
A Novel 10 Layer Flask For use in the SelecT Automated Cell Culture System
The new Corning HYPERFlask vessel is designed and optimized to increase cell yield using the SelecT Automated Cell Culture System. Importantly, cell densities per unit growth area are similar to traditional single vessels resulting in approximately ten times the cell yield as traditional cell culture vessels in the same footprint. The cells grown in the HYPERFlask perform exceptionally well in cell based assays.
Poster
Peptidic and Non-Peptidic Ligand Activated GPCRs-Comparison of Assay Platforms
A number of GPCRs activated by peptide ligands were assessed using different assay technologies. It was observed that the Ec50 values obtained using htrf assays were significantly higher than those obtained with other assay platforms. This difference was not observed with a non-peptide liganded GPCR. This highlights that with the GPCRs tested the use of htrf technology may not be the most appropriate assay format for peptide liganded GPCRs.
Poster
Transcreener™ PDE Assay: Homogenous AMP and GMP Detection
To facilitate the rapid identification of PDE inhibitors, BellBrook Labs has developed a fluorescence polarization immunoassay that directly detects AMP and GMP. The Transcreener™ PDE Assay includes a single set of reagents - an anti-AMP/GMP antibody and fluorescent tracer - that can be used for any cyclic nucleotide PDE, providing a robust, cost-effective alternative to currently available technologies.
Poster
An Emerging Revolution in Automated 3D Cell Culture
Market pressure exists for new paradigms to meet the demands for more rapid and reproducible cell production to keep up with advances in cell-based assays used in drug discovery and development. Novel automation platforms enable high productivity on three dimensional surfaces.
Poster
Novel Fluidics Microbead Trap/Flow Cell Enhances Speed/Sensitivity of Bead-Based Bioassays Up to 5-Fold
Pacific Northwest National Laboratory (PNNL) has developed a micro/nano particle trap that allows surface-functionalized magnetic or non-magnetic particles to be trapped with subsequent perfusion of sample, reagents and wash solutions, yielding significant (up to 5-fold) improvements in assay speed and sensitivity, while significantly reducing sample matrix effects.
Poster
Temporal Dynamics of Pathogenesis Related Metabolites and their Metabolic Pathways Following Inoculation of Potato Leaves with Phytophthora infestans
The resistance in potato cultivars against late blight caused by Phytophthora infestans is classified into vertical and horizontal. Several metabolites associated with resistance have been detected: phytoalexins such as rishitin, phytuberin, lubimin, solavetivone etc. Metabolite profiling is a novel technology for the discrimination of quantitative resistance in plants against pathogen stress.
Poster
Peform Faster Proteomics with ProtE
In the present paper we describe results obtained using a miniaturized ProtE instrument and its comparison to the mini- PROTEAN 3-cell (Bio-Rad). Our device is able to perform sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) in less than 11 min.
Poster
Towards Understanding of Ryegrass-Endophyte Symbiosis through Data Integration of Transcriptome and Metabolome
Combined with extensive bioinformatics analyses, our joint analysis of transcriptomics and metabolomics data has generated interesting findings which led to further laboratory verification, such as potential genes associated with alkaloid production.
Poster
Indirubin inhibitors of Leishmania mexicana CRK3 Cyclin Dependent Kinase
The indirubins are known inhibitors of cyclin dependent kinases (CDKs), including CRK3 from the protozoan parasite Leishmania mexicana, which is essential for proliferation of the disease Leishmaniasis. Subsequent investigations into the pharmacological properties of the indirubins show them to be competitive ATPbinding site inhibitors of the CDKs.
Poster
Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions
In this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation.
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