Latest Posters
Poster
A High Content Primary Screening Assay Determining ERK and JNK Activation
Here we present a rapid method for determining ERK and JNK activation, demonstrated using time course, concentration-dependence data and the effect of the MEK1/2 inhibitor UO126 on ERK activation obtained using the Acumen Explorer™ laser scanning cytometer analysing 384 well plates.
Poster
Cell Cycle Analysis Using Microplate Cytometry: A Comparison of Laser and Dye Combinations
For improved screening capability, we have developed a cell cycle analysis method using an Acumen Explorer fluorescence microplate cytometer, capable of reading an entire 384 well microplate in about 10 minutes. The method can perform such analyses on fixed cells in situ, markedly simplifying sample preparation.
Poster
Detection of BD Living Colors™ Novel Fluorescent Proteins Using the Acumen Explorer™
Proprietary Novel Fluorescent Proteins (NFP) from BD Biosciences Clontech include fluorescent proteins cloned from reef corals and the jellyfish Aequorea coerulescens. Fluorescent emissions of distinct proteins from this portfolio were analysed by the Acumen Explorer.
Poster
Determination of Cell Colony Formation in a High Content Screening Assay
Here, laser scanning microplate cytometry has been used to provide an automated high content readout of the effects of cytostatic agents on colony formation. This approach determines colony number through the application of a spherical volume algorithm. This permits the differentiation of cytostatic effects where the number of colonies and size remains constant and cytotoxic effects where the size and number may be reduced.
Poster
Turning Valves for Lab-on-a-Chip Applications Enable Directional Flow and Portion Out Pre-Defined Volumes
In this study the design and the realization of low-cost valves suitable for the integration into disposable polymer chips is presented. In contrast to commonly used sandwich-setups a plain design consisting of a simply structured polymer chip and a cover membrane is used.
Poster
A Valve-Controlled Microfluidic Sysytem for Rapid Two-Dimensional Electrophoresis
Two-dimensional electrophoresis of proteins is achieved in 500 seconds in a microfluidic system fabricated from PDMS, in which the first dimension of micellar electrokinetic chromatography and the second dimension of capillary sieving electrophoresis are coupled. By installing valves at the intersection of two dimensions, the separations are performed sequentially without interference.
Poster
Small Volume Liquid Transfer Technology
High reagent costs associated with molecular biology assays are driving trends toward continued volume reduction and parallelization of assays. Several robotic pipettors are available for small-volume, multi-well pipetting, but suffer from some disadvantages. We present two projects to address some of the drawbacks.
Poster
Polymer-Bound Fused N -(1-Chloroalkyl)Azinium Chlorides As Synthetic Equivalents Of Aldehydes.
A few years ago the first multi-component synthesis of N-(1 -haloalkyl) pyridinium halides from a thionyl halide, pyridine, and an aldehyde was described. Since that time, we have widely demonstrated that such salts can be considered as valuable synthetic equivalents of the starting aldehydes for the preparation of various classes of nitrogen heterocycles.
Poster
Assay Miniaturisation using Hummingbird and synQUAD Systems
The data shown demonstrates that assay integrity is maintained when dispensing nanoliter volumes to 1536 well plates. Significant savings in reagent and assay costs can be realised from assay miniaturisation with the additional advantage of getting more assays and data from compound stocks before depletion.
Poster
A Flexible Solution for High Speed Sample Cherry-picking from Frozen Storage
The comPOUND® sample store (TTP LabTech) is a modular and flexible system that can be installed almost anywhere. This fully scaleable approach to compound storage also allows sample throughputs to increase with the library size to match process needs.
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