The Use of Software Visualization Tools for the Identification of PTM Patterns and Artefacts in LC-MS Experiments
By representing LC-MS data as a 2D-image as demonstrated in the novel DeCyder™ MS software, it is possible to identify phenomena, which are very difficult to recognize using conventional LC-MS data representation.
Sensitive Identification of Phosphopeptides in Brain Tissue using 2D-NanoLC-ESI-MSn
In this work, a biocompatible nano liquid chromatography (LC) system, Ettan™ MDLC, was used for separating tryptic peptides from brain tissue by cation exchange (SCX) to enrich the phosphopeptides followed by reversed-phase chromatography (RPC). The phosphopeptides were detected by neutral loss MS.
IGF1 Biomarker Assay Validation
This work demonstrates the development and validation of a novel clinical proteomics approach in which specific protein targets can be expeditiously and selectively isolated from acomplex biological fluid for MS characterization.
Addressing the Repoducibility Aspect of LC-MS Based Protein Identification
By using the detection, matching, and visualization approach ofDeCyder™ MS were retention time, precursor mass, and the topology of the intensity profile is utilized in combination with the matching of tandem mass spectra, it is possible to achieve repeat analysis with a very high reproducibility.
Functional Characterization of the Human Fractalkine Receptor CX3CR1 Using Photina™, a new Highly Sensitive Photoprotein
CX3CR1 has been stably transfected into CHOmitoPhotina™- MRE-CRE-Luciferase cell line and assays suitable for HTS and uHTS have been developed.
GPVI-RBL-2H3-NFAT-Re Luciferase Cell Line: a New Cell Based System for Studying Collagen Receptor Activation
This study describes the generation of a cell based assay which allows the functional characterization of the human GPVI receptor, by the use ofluminescence.
High Density Receptor Ligand Binding Assays in the MultiScreen®HTS 384-well Glass Fiber Filter Plate
Using the Muscarinic M1 G-protein Coupled Receptor as a model system, we achieved accurate and reproducible determinations of binding affinity (Kd) and IC50 results for known ligands on the 384-well plate device. As a result of being able to incubate the reaction mixture in the filter plate and configure the assay using half the reaction volume, significant reductions in reagent costs and radioactive waste were achieved.
Screening of Biologically Active Natural Products in PIBOC
We screen natural products for antimicrobial, antiviral and anticancer activity, immunomodulatory effects, as inhibitors of cell adhesion and antioxidants. High resolution MRI-tomography with 7T magnet (PharmaScan 70/16US, Bruker) allows us to search in situ for compounds with anticancer, anti stroke and anti ischemia activity, inhibitors of angiogenesis and hepatoprotective substances.