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Latest Posters

MicroRNA Expression Signature in Human Glioblastoma Multiforme Brain Tumor content piece image
Poster

MicroRNA Expression Signature in Human Glioblastoma Multiforme Brain Tumor

Expression of 180 human miRNAs was examined using recently developed stem loop primers for reverse transcription (RT) followed by real-time PCR. MicroRNAs can be quantified from as few as single cells or as little as 25 pg total RNA.
rptDB; a prototype Affymetrix .rpt QC tool content piece image
Poster

rptDB; a prototype Affymetrix .rpt QC tool

rptDB is a prototype database for the analysis of Affymetrix expression report (.rpt) files. Report files are widely used to QC raw data after hyb, scan and .cel file generation. Only %Present calls, Scale Factors and 3':5' ratios of housekeeping genes are generally used, but there are many other metrics that may also be of importance for QC analysis.
Development of low Cost High Throughput miRNA Screening content piece image
Poster

Development of low Cost High Throughput miRNA Screening

Micro-RNAs (miRNAs) form a class of 17-24 base singles trandnon-coding RNAs which regulate gene expression post-transcriptionally in eukaryotes and viruses. We have developed a low cost high throughput method to quantify the expression of these miRNAs via classictotal RNA extraction without interference from genomic DNA.
A Mild Protocol for Parallel Solution Phase Synthesis of Cyclic Imides  content piece image
Poster

A Mild Protocol for Parallel Solution Phase Synthesis of Cyclic Imides

The combination of using high temperatures and coupling reagents allows production of cyclic imides rapidly and in higher purity than heat or coupling agents alone.
Microwave-Accelerated Fischer Glycosidation content piece image
Poster

Microwave-Accelerated Fischer Glycosidation

Fischer glycosidation appeared an ideal candidate for the application of microwave irradiation and herein we report a systematic analysis of the effect of microwave heating in the Fischer glycosidation reaction.
Chip’N’Fish : Novel Chemical Proteomics Platform for Rapid Identification of Small Molecule Drug Candidates Against Disease-Specific Targets content piece image
Poster

Chip’N’Fish : Novel Chemical Proteomics Platform for Rapid Identification of Small Molecule Drug Candidates Against Disease-Specific Targets

Over-expression of genes and their protein products is often the best starting point for dissecting the pathways that are responsible for the development of specific diseases.The Chip'N'Fish approach allows identifying over-expressed proteins directly from cell extracts of disease origin using healthy counterparts as controls.
High-Throughput Automation of a Dual Reporter Assay in Low Volume 384 & 1536 Well Plate Formats  content piece image
Poster

High-Throughput Automation of a Dual Reporter Assay in Low Volume 384 & 1536 Well Plate Formats

The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator™ NS 808 - Eight Tip Pipetting System and the dual color Chroma-Luc™ technology.
Miniaturisation of Liquid Handling Procedures for High Throughput Sequencing  content piece image
Poster

Miniaturisation of Liquid Handling Procedures for High Throughput Sequencing

Deerac Fluidics Equator™ products are designed to fit a wide range of applications requiring low volume liquid handling. The products have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA. This poster will describe the current process employed by Broad Institute, highlighting where the Equator™ has played a vital role in performance enhancement and cost reduction, enabling the Broad Institute to retain its position as
Software for Automated Differential Expression Using 2D and 3D Representations of LC-MS Data Interactive Confirmation of Results content piece image
Poster

Software for Automated Differential Expression Using 2D and 3D Representations of LC-MS Data Interactive Confirmation of Results

DeCyder™ MS is a novel software program for fully automated differential expression analysis based on LC-MS/MS data including detection, quantitation, sample to sample comparison and statistical data evaluation. Results can be interactively confirmed against original raw data through 2D and 3D data visualizations.
The Use of Software Visualization Tools for the Identification of PTM Patterns and Artefacts in LC-MS Experiments content piece image
Poster

The Use of Software Visualization Tools for the Identification of PTM Patterns and Artefacts in LC-MS Experiments

By representing LC-MS data as a 2D-image as demonstrated in the novel DeCyder™ MS software, it is possible to identify phenomena, which are very difficult to recognize using conventional LC-MS data representation.
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