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Micro-RNAs (miRNAs) form a class of 17-24 base singles trandnon-coding RNAs which regulate gene expression post-transcriptionally in eukaryotes and viruses. We have developed a low cost high throughput method to quantify the expression of these miRNAs via classictotal RNA extraction without interference from genomic DNA.

The combination of using high temperatures and coupling reagents allows production of cyclic imides rapidly and in higher purity than heat or coupling agents alone.

Fischer glycosidation appeared an ideal candidate for the application of microwave irradiation and herein we report a systematic analysis of the effect of microwave heating in the Fischer glycosidation reaction.

Over-expression of genes and their protein products is often the best starting point for dissecting the pathways that are responsible for the development of specific diseases.The Chip'N'Fish approach allows identifying over-expressed proteins directly from cell extracts of disease origin using healthy counterparts as controls.

The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator™ NS 808 - Eight Tip Pipetting System and the dual color Chroma-Luc™ technology.

Deerac Fluidics Equator™ products are designed to fit a wide range of applications requiring low volume liquid handling. The products have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA. This poster will describe the current process employed by Broad Institute, highlighting where the Equator™ has played a vital role in performance enhancement and cost reduction, enabling the Broad Institute to retain its position as

DeCyder™ MS is a novel software program for fully automated differential expression analysis based on LC-MS/MS data including detection, quantitation, sample to sample comparison and statistical data evaluation. Results can be interactively confirmed against original raw data through 2D and 3D data visualizations.

By representing LC-MS data as a 2D-image as demonstrated in the novel DeCyder™ MS software, it is possible to identify phenomena, which are very difficult to recognize using conventional LC-MS data representation.

In this work, a biocompatible nano liquid chromatography (LC) system, Ettan™ MDLC, was used for separating tryptic peptides from brain tissue by cation exchange (SCX) to enrich the phosphopeptides followed by reversed-phase chromatography (RPC). The phosphopeptides were detected by neutral loss MS.

This work demonstrates the development and validation of a novel clinical proteomics approach in which specific protein targets can be expeditiously and selectively isolated from acomplex biological fluid for MS characterization.