2nd round audit of skeletal surveys in patients with Monoclonal Gammopathy of Undetermined Significance (MGUS)
Poster Aug 29, 2014
Dr Ganesh Retnasingam, Dr Laura Day and Dr Yvette Griffin
To ensure British Journal of Haematology guidelines are being followed by the haematologists at University Hospitals of Leicester and no unnecessary requests for skeletal surveys in MGUS as per agreed action plan following results from 1st round audit.
Results of 1st round audit from 16/10/2006 to 16/12/2011 showed 14% of 88 MGUS patients had a skeletal survey when not indicated. This is because they had a paraprotein <10g/l and no other risk factors.
We retrospectively reviewed consecutive patients from the myeloma MDT database, newly diagnosed with MGUS, between May 2013 to May 2014.
From ilab we obtained paraprotein type/level, serum kappa/lambda ratio, renal function, full blood count, B2 microglobulin, serum calcium, albumin, urine Bence-Jones Protein. If any of these were abnormal they were considered risk factors for progression to myeloma.
24 patients: 8 male, 16 female. Average age 72, age range 49 to 92. Only 1 patient had skeletal survey when not indicated with a serum paraprotein level of 2.2g/l and no risk factors.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE