3D-Tissue/ Whole-blood Co-culture Models Combined with Multi-Analyte Profile (MAP) Analyses for In-vivo-like Immunopharmacology
Poster Feb 25, 2015
Stein GM, Joos T, Schmolz M
The cross talk between immune cells and tissue cells controls inflammation and is mediated by mediators such as chemokines, cytokines and others. This network of signals can be modulated therapeutically by drugs.
The effect of drugs on such omplex regulation can be analysed reliably only in an environment that represents the complexity of such feedback systems. HOT Screen GmbH developed a series of proprietary human organotypic co-culture test systems combining whole-blood cultures with cultures of differentiated intestinal epithelia, 3D epidermis, bronchial epithelia, etc. These allow to study drug effects on immunoregulation even in in-vivo-like human inflamed tissue environments.
Multiplexed Luminex® assays (MAP tests, MyriadRBM) were used as complex readouts when testing drug effects on cell activation.
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE