A 1H-NMR Based Metabonomics Study of Urine and Plasma Obtained from Healthy Human Subjects
Poster Feb 23, 2006
E.M. Lenz, J. Bright, I.D. Wilson, S.A. Morgan and A.F.P.Nash
IntroductionMetabonomics is well established as a means of disease and toxicity screening in experimental animals.
Metabonomics will find increased application in the study of healthy and diseased humans. However, one of the major obstacles in clinical investigations is the greater variability in a human population.
Here, we describe an investigation on the plasma and urine samples of healthy male volunteers designed to evaluate the variability in metabonomic data, when severe life-style and dietary restrictions are imposed.
We developed an automated sample preparation protocol based on a robotic platform PAL RTC (CTC Analytics AG, Zwingen Switzerland), which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics using SFC-MS simultaneously.READ MORE