A Flexible Solution for High Speed Sample Cherry-picking from Frozen Storage
Poster Mar 02, 2006
Jas Sanghera, Ben Schenker, Simon Tullett, Chloë Milburn
AbstractTo successfully automate sample storage and retrieval, compound samples must be quickly and reliably delivered to the location where they are required. Implementing the store itself should not cause a company major upheaval and reorganisation, and – especially important for smaller companies – the store must be able to grow with a company’s business.
The comPOUND® sample store (TTP LabTech) is a modular and flexible system that can be installed almost anywhere. This fully scaleable approach to compound storage also allows sample throughputs to increase with the library size to match process needs.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE