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A microfluidic approach for the directed evolution of proteins by retroviral display
Poster

A microfluidic approach for the directed evolution of proteins by retroviral display

A microfluidic approach for the directed evolution of proteins by retroviral display
Poster

A microfluidic approach for the directed evolution of proteins by retroviral display

The model system used here is based on retroviral particles displaying tPA, a protein used in current emergency therapies of myocardial infarction and stroke. Single tPA variants were encapsulated into aqueous droplets, at a frequency of ~10Kilohertz and the enzymatic activity was monitored using a fluorescence assay. Active variants could be clearly distinguished from inactive variants or variants incubated with the endogenous inhibitor PAI-1.
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