A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications
Poster Apr 15, 2015
Gurkan Yesiloz, Muhammed S. Boybay, Carolyn L. Ren
We report the development of a droplet-based microfluidic device integrated with a microwave sensor and heater for simultaneous sensing and heating of individual nanoliter-sized droplets. it is demonstrated that droplets can be heated up more than 40 degrees above room temperature within milliseconds. For sensing performance, we validated that this microwave sensor can differentiate various different materials with subtle difference in their electrical properties (electrical conductivity and/or dielectric constant) at magnitude higher throughput.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE