A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins
Poster Dec 05, 2013
Antti Vasala, BioSilta Oy, Oulu, Finland
Most frequently, bacteria are grown in shake flasks or plates using commercially-available or ‘in-house’ adaptations of LB, TB or M9. Ehen supplied with a carbon source such as glucose, bacteria grow and divide rapidly at their maximum growth rate. However, as uncontrolled growth and fast aerobic metabolism deplete oxygen and glucose levels and alter the surrounding pH, bacteria soon switch over to anaerobic metabolism.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE