A Novel Array-Based Assay for the Detection of IgG-Mediated Food Intolerance
Poster Nov 08, 2006
Andrew Macdonald, Michael J. Walker, Michael S. Walker and Julie G. Reeve
IgG reactions to food have been implicated in a number of chronic diseases including arthritis, irritable bowel syndrome, bronchitis and depression. Such reactions involve the formation and deposition of antigen/antibody complexes in a variety of tissues where they cause inflammation, pain and other symptoms.
The measurement of food-specific antibodies by microplate-based immunoassays is currently used for the assessment of these reactions and several studies have shown that food elimination diets, based on food IgG determinations, often help resolve symptoms. Consequently, the demand for food IgG measurements has increased world-wide and, in acknowledgment of this, we have developed a microarray-based immunoassay to permit both greater food panel diversity and higher throughput testing.
The Genarrayt™ 200+ Foods IgG test comprises of glass slides onto which 16 microarrays of over 200 different foods have been printed. Each microarray includes standards for quantitation and positive and negative controls for quality control. Food IgGs are detected by a novel fluorescent dye labelled anti-human IgG conjugate and results are measured using a laser scanner. Fluorescence intensity is directly proportional to antibody activity in the sample. The assay has a number of advantages over conventional ELISA including increased sample throughput, small reagent volumes and much greater information.
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