A Novel Benchtop Time-of-Flight GC-MS System for High Performance Analysis of Human Urine
Poster Sep 13, 2016
David E. Alonso, Joseph E. Binkley, Lorne M. Fell
The development of improved profiling workflows are critical for effective investigation of environmental exposure and its relationship to chronic diseases. Metabolomic methods are particularly useful in this endeavor due to the proximity of metabolites to system phenotype, and their relatively quick insight into system perturbations from diet, drugs, gut microbiota, etc. (Figure 1). Unfortunately, a major problem with these methods continues to be incomplete sample characterization due to metabolite diversity, wide concentration range, and the overall complexity of biological matrices.
Urine is an ideal bio-fluid for studying the effects of exposure, since it is easy to obtain in large volumes, and is relatively free from interfering proteins and lipids. In addition, high concentrations of drugs and other xenobiotic materials can be detected over extended periods of time. In this study, we used a high performance, benchtop gas chromatography time-of-flight mass spectrometer for comprehensive characterization of derivatized urine samples (Figure 2).
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.