A Novel Cell-Free DNA Extraction System for Cancer Diagnostics
Poster Oct 14, 2016
Amanda Fan, Alex Yeo, Yin Kum Ng, Elian Rakhmanaliev, Pramila Ariyaratne, Charlie Lee, Gerd Michel, Tatiana Ivanova
Circulating cfDNA in human plasma becoming an important source for tumour mutation detection in cancer diagnostics. In this study we compared a column-based method and a novel automated magnetic beads-based kit (Sentosa SX cfDNA Kit (4x8)). This kit was optimized for use on a robotic liquid handling platform (Sentosa SX101). Quality of cfDNA extracted by both methods was assessed using ALU repeats qPCR assay and NGS-based Sentosa SQ CRC test. DNA was extracted from plasma samples with spiked-in fragmented (~170bp) HCT116 gDNA (KRAS G13D positive) using both methods. The ALU247/115 ratio for DNA extracted by the Sentosa kit was 0.19-0.28 and for the column-based method >0.7 (expected ratio for cfDNA <0.5 and for gDNA ~1.0). Amount and quality of DNA extracted by both methods was sufficient to prepare NGS libraries for all samples. KRAS G13D was detected in all samples extracted by the Sentosa kit but not in column-based extracted samples. The Sentosa cfDNA kit selectively extracts cfDNA over high molecular weight gDNA and appears as an efficient solution for cfDNA extraction from plasma. Integration into the qPCR- and NGS-based workflows makes the Sentosa cfDNA kit a universal diagnostics tool, which can be used in combination with various IVD assays.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Correlations among the Toddler Gut Microbiome and Health Status VariablesPoster
Establishment and succession of the human microbiome begins at birth and microbial composition adapts alongside human development and growth. Development of a healthy signature microbial community has significant effects on health.This study followed 18 very low birth weight (VLBW) infants, measured initially in the from Neonatal Intensive Care Unit (NICU), at the age of two to three years old.READ MORE
A new method for generating arrayed RNAi screening tools for any organismPoster
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE
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