A novel Hydrogel Mountant - CyGEL™ - Enables Temporospatial HCS Imaging Assays of Live non-Adherent Cells
Poster Apr 25, 2007
Edward R, Errington RJ, Patterson LH, Ogrodzinski S and Smith PJ
Imaging-based assays have primarily relied upon adherent cell types for temporospatial measurements. This limits choice of cell type and extension of detection technologies to relevant biological models that may comprise nonadherent (e.g. suspension culture) or indeed ‘detaching’ (e.g. loss of viability/adherence) cell states such as drug candidate screening in immunological and lymphoproliferative disorder models, where the therapeutic target is often presented by a non-adherent type cell or where a biological response requires changes in adherence to a substrate. New technologies are thus needed to deal with live cell-based HCS assays on non-adherent cells or to exploit suspension cells in fluidic systems.
Here we describe CyGEL™ - a thermo-reversible hydrogelbased 4-D immobilization technology for live cell location without imposing an anchoring to a substrate.