A Novel Multi-organ Microfluidic Chip: On the Way to the Complexity of a Living Organism
Poster Mar 17, 2015
Timur R Samatov, Svetlana A Tonevitskaya, Natalya Pulkova, Evgeny A Tonevitsky
Currently an increasing number of physiologically relevant organ-on-chip platforms are reported. Most of them are focused on modeling particular organs or their functional elements. Here we present H-chip, a novel platform capable of culturing up to six different organotypic models integrated into a single microfluidic circuit. The developed platform provides constant long-term circulation and automated replenishment of medium in the circuit. The key characteristics of the medium flow, including volumetric flow velocity and resulting shear stress, are similar to the ones found in a human organism. The medium flow-dependent increased cell viability of cultured cells supports the physiological relevance of the presented platform.
Spinal muscular atrophy (SMA) is an inheritable cause of infant mortality that is characterized by the loss of lower motor neurons and skeletal muscle atrophy. The degeneration of motor neurons is caused by insufficient levels of survival motor neuron (SMN) protein, which is encoded by two nearly identical genes SMN1 and SMN2. Most cases of SMA harbour homozygous deletions of the SMN1 gene and retain at least one copy of SMN2.READ MORE