A Novel Multi-organ Microfluidic Chip: On the Way to the Complexity of a Living Organism
Poster Mar 17, 2015
Timur R Samatov, Svetlana A Tonevitskaya, Natalya Pulkova, Evgeny A Tonevitsky
Currently an increasing number of physiologically relevant organ-on-chip platforms are reported. Most of them are focused on modeling particular organs or their functional elements. Here we present H-chip, a novel platform capable of culturing up to six different organotypic models integrated into a single microfluidic circuit. The developed platform provides constant long-term circulation and automated replenishment of medium in the circuit. The key characteristics of the medium flow, including volumetric flow velocity and resulting shear stress, are similar to the ones found in a human organism. The medium flow-dependent increased cell viability of cultured cells supports the physiological relevance of the presented platform.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE