A Novel Multi-organ Microfluidic Chip: On the Way to the Complexity of a Living Organism
Poster Mar 17, 2015
Timur R Samatov, Svetlana A Tonevitskaya, Natalya Pulkova, Evgeny A Tonevitsky
Currently an increasing number of physiologically relevant organ-on-chip platforms are reported. Most of them are focused on modeling particular organs or their functional elements. Here we present H-chip, a novel platform capable of culturing up to six different organotypic models integrated into a single microfluidic circuit. The developed platform provides constant long-term circulation and automated replenishment of medium in the circuit. The key characteristics of the medium flow, including volumetric flow velocity and resulting shear stress, are similar to the ones found in a human organism. The medium flow-dependent increased cell viability of cultured cells supports the physiological relevance of the presented platform.
Primary Human Hepatocyte (PHH) culture provides the closest in vitro model to human liver that can produce a metabolic profile of a given drug very similar to that found in vivo. Recently we have developed an easy-to-assemble user-friendly in vitro Primary Human Hepatocyte (PHH) 3D-spheroid modelREAD MORE