A Solution for Low Volume Pipetting Applications Requiring High Accuracy of Sample Placement
Poster Feb 27, 2006
Joby Jenkins, Rob Lewis, Tristan Cope and Chloë Milburn
The mosquito® nanolitre pipettor (TTP LabTech) uses precise, stepper motor driven, linear drives in conjunction with optical sensors to achieve positional accuracy better than 0.05 mm in the X, Y and Z axes. This, in conjunction with positive displacement, disposable pipettes which guarantee zero cross-contamination, gives the mosquito® unrivalled accuracy and repeatability for these more complex transfer operations.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE