A Solution for Serial Dilution of Compounds in 1536-Well Microplates
Poster Feb 27, 2006
Rose Hughes, Jose Quiroz, Joe Zer, Robert Bukar, Joby Jenkins, Wayne Bowen
Based around 1536-well microplates and now in its third generation, the Kalypsys Systems uHTS System has a throughput of over 1 million assay wells per day, and an on-line storage capacity of over 2.2 million compounds. The focus on 1536-well miniaturization has created a need for liquid handlers that are capable of accurately and reliably transferring and diluting small volumes of liquid in 1536-well plates. Kalypsys has identified the TTP LabTech mosquito® as apractical and cost-effective solution for 1536-well low volume serial dilution.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE