A Specific Immunocapture Activity Assay for Matrix Metalloproteinases Using 5-FAM/QXL 520TM FRET Peptide
Poster Jan 16, 2007
Yi Tang, Xiuqing Li, Xudong Zhu, Xin Han, and Zhenjun Diwu
Addressing the limitations of previous FRET-based MMP assays, this poster documents the development of immunocapture-based FRET assays that are highly sensitive and specifically detect the activity of a particular MMP in a mixed biological sample. The assay sensitivity can reach picogram amounts of enzyme with a large and linear assay range. The assay can also be used to study the interaction between different MMPs.