A Total Solution to Provide High Content Primary and Secondary Screening of a Compound Library
Poster Feb 01, 2007
Ben Schenker, Silja Yates and Ian Yates
G-protein coupled receptors (GPCRs) represent the largest and most frequently screened class of receptors. TTP LabTech recently published a novel approach using the Acumen Explorer with Invitrogen’s GeneBLAzer technology to measure GPCR activation in cell-based assays. This is multiplexed with a cytotoxicity readout to reduce false positives commonly identified in bulk fluorescence assays. TTP LabTech and Velocity11 have designed a fully automated, single platform using this assay to perform both primary and hit-confirmation screens on a 400,000 compound library in only one week.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE