A Total Solution to Provide High Content Primary and Secondary Screening of a Compound Library
Poster Feb 01, 2007
Ben Schenker, Silja Yates and Ian Yates
G-protein coupled receptors (GPCRs) represent the largest and most frequently screened class of receptors. TTP LabTech recently published a novel approach using the Acumen Explorer with Invitrogen’s GeneBLAzer technology to measure GPCR activation in cell-based assays. This is multiplexed with a cytotoxicity readout to reduce false positives commonly identified in bulk fluorescence assays. TTP LabTech and Velocity11 have designed a fully automated, single platform using this assay to perform both primary and hit-confirmation screens on a 400,000 compound library in only one week.