A Workflow for Comprehensive Analysis of Forensic Samples Using Electron and Chemical Ionization High Resolution Time-of-Flight Mass Spectrometry
Poster Oct 30, 2014
David E. Alonso, Joe Binkley, and John Rorabeck
Controlled substances such as marijuana, cocaine, heroin and methamphetamines are commonly seized by law enforcement officials and routinely analyzed by forensic laboratories. More recently, synthetic drugs such as bath salts and cannabinoids, as well as naturally occurring “herbal remedies” and hallucinogenic mushrooms, have gained popularity as chemical stimulants and opiate substitutes. This study focuses on the comprehensive profiling of complex botanicals obtained from drug seizures.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE