Accurate Determination of Moisture Content of Soft Contact Lenses by Near-Infrared (NIR) Spectroscopy
Poster Mar 03, 2014
Dr. Hari Narayanan, Dr. Keith Freel
Near infrared (NIR) spectroscopy is a rapid, non-destructive method for analysis of routinely measured products. NIR can eliminate lost time and lost product while improving real time quality control. The delicate properties of soft contact lenses require a minimal-contact method for analysis to avoid damage to the lens itself. NIR spectroscopy provides a quick and damage-free solution. Four sets of soft contact lenses with known moisture levels were analyzed by NIR spectroscopy. The spectra were analyzed for quantitative model development. Difficulties arose due to moisture loss by evaporation. However, with careful attention to testing procedures, a model was successfully developed and used to determine the embedded moisture levels in soft contact lenses with an error less than 1 %. Several methods were tested and evaluated based on accuracy, precision, experimental simplicity, and potential for lens damage.
Plasmodium falciparum is evolving resistance to Artemisinin Combination Therapy. The gene with the strongest association with resistance is K13. K13 is an ortholog of the well characterized transcriptional regulator Keap1. In this work we transcriptionally characterized a mutant with a transposon inserted in the K13 promoter region which results in dysregulation of K13 at 2 points of the intraerythrocytic cycle of the life-cycle to identify the processes regulated by K13.READ MORE
PfABCk2 gene is ligated into pET21a+ vector with His-tag at C-terminus and transformed into BL-21 (DE3) competent cells that are verified through Miniprep and DNA sequencing. Furthermore, this gene construct is utilized to heterologous express this protein with IPTG and afterwards purified the recombinant protein kinase using nickel affinity chromatography as shown on 10% SDS-PAGE with the expected 36 kDa protein band.READ MORE
8th Edition of International Conference and Exhibition on Separation Techniques
Jul 29 - Jul 30, 2019