Achieving Ultra High Performance Screening
Poster Jan 10, 2007
David James Morris, Steve Mayhew and William Hoyland
High throughput (HT) and Ultra High Throughput (UHT) screening remains a valuable and necessary approach to modern drug discovery. With the use of numerous standardized biochemical assays now commonplace, successful application of HTS requires accurate and meaningful handling of the experimental results. Specifically, this means capturing vast data volumes generated by HTS and UHTS programmes and subsequent validation and verification. Decision making regarding HTS data can be supported via comprehensive and intuitive visualization of results. Also important is the establishment and maintenance of analysis templates for this type of experiment. In order for HTS and UHTS to be successfully implemented in drug discovery, all the aforementioned issues must be addressed.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE