Acoustophoretic Microfluidic Device for High Throughput DNA Sequencing
Poster May 29, 2015
V.V Unnikuttan1, H.N Unni 1
Modern DNA sequencing technique is a cumbersome, expensive and vast lab process and our aim is the miniaturization of the huge lab process which brought about the inception of the device known as “lab-on-a-chip” (LOC). Acoustophoresis is the ultrasound-induced control of the motion of particles in the microfluidic channel which is rapidly becoming a popular technology in the modern LOC devices.
The factors that affect particle motion in the channel on application of acoustic wave are mainly volume of particle, compressibilities and densities of particle and medium and the acoustic pressure applied which depends on the acceleration of the piezo- crystal. Acoustophoretic transport can be modeled by Continuity equation, Navier-Stokes equation, and Convection – Diffusion equation. The resonant frequency of piezo- crystal corresponding to maximum pressure or acceleration, depends on the piezo- material, size and voltage applied to it. The present study focuses on the optimization of acoustic parameters and simulation of particle transport using COMSOL.
Acoustic standing wave technology combined with micro technology opens up new areas for the development of advanced particle separating microfluidic systems with reasonable throughput and ability to separate particles that helps us to make an automated device for preparing purifying and analyzing DNA samples.
The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.READ MORE