Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics
Poster Mar 14, 2016
Shubha Jain, F. Azam, Dr. H. N. Unni
We have demonstrated a microfluidic mixer device to characterized and study the macromolecule dynamics such as kinetics of protein folding, DNA/RNA sequencing, single molecule study and detection etc. Furthermore by integrating the concept of microfluidic mixer with magnetic beads, make feasible to study the next-generation sequencing applications like genomic DNA sequencing of microbial species from single cell and protein RNA interaction. We applied the concept of geometric variation such as length, height, geometric shape and number of channel to enhance the mixing efficiency without applying any external force or energy called passive mixing. To control the viscous force low Reynolds number is considered, thus the flow would be laminar. As a result we achieved the complete mixing at the latest reservoir. The Comsol Multiphysics platform has been used to study the simulation work by coupling of convective-diffusion and Navier-Stokes equation. In addition, applications of microfluidics technology use in Lab on chip devices and also span biomedical and healthcare field enormously.
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Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE