Alzheimer's Disease Model Cells Derived from Human iPS Cells
Poster Mar 20, 2015
Honda M; Kitogo T; Kogami H; Inamura M
Alzheimer's disease (AD) is the most common cause of dementia characterized by impaired memory and cognitive dysfunction due to neurodegeneration. The predicted increase in AD morbidity coupled with the socioeconomic impact of the disease has necessitated the urgent development of an effective therapy. However, the currently available models of AD are challenging and a more humanized, scalable assay system is required to better understand the disease and identify novel therapies.
There are two methods to develop AD model cells based on human induced pluripotent (iPS) cells. The first method uses targeted genetic modification of human iPS cells, and the other involves the production of human iPS cells from patients with AD. We supply both types of AD model cells depending on request.
In this poster, we describe how we developed the AD model cells (ReproNeuro Ach-ADTM and ReproNeuro Glu-ADTM ) by generating human iPS cells containing a mutant Presenilin 1 (PS1) gene, which is responsible for familial AD, and differentiating these cells into cholinergic neurons or glutamatergic neurons. In the brains of patients with AD, cholinergic neurons are damaged and the glutamate system is disrupted. To confirm whether these neurons can be used for drug screening, we performed characterization of these neurons as well as the Aβ assay. The effect of these neurons on the production of Aβ40 and Aβ42 was measured using the AlphaLISA® Human Amyloid β1-40/1-42 immunoassay kit (Perkin-Elmer) for high-throughputscreening (HTS).
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
Quantitative Live-Cell Analysis Using Automated Long-Term ImagingPoster
Here we describe a continuous live-cell assay for determining cell proliferation profiles using the BioSpa Automated Live Cell Imaging System, consisting of BioSpa 8 and Cytation 5.READ MORE
Assessment of Oral LISPRO Treatment in Ameliorating Amyloid and Tau Pathology in Transgenic Alzheimer’s Mice ModelPoster
Ionic co-crystals of lithium salicylate with organic proline (LISPRO) showed better safety and pharmacokinetic profile of lithium in plasma and brain of wild-type and transgenic Alzheimer mice model compared to lithium salts.READ MORE