Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection
Poster Oct 06, 2014
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn
Water-soluble derivatives of gadolinium-containing metallofullerenes have been synthesized and tested as excellent candidates for magnetic resonance imaging (MRI) contrast agents because of their high relaxivity and robustness. It is expected that nanoparticles with a positive charge will bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. Although numerous gadolinium-containing metallofullerenes diagnostic MRI contrast agents have been reported, the metallofullerene cage surface in most cases consist of negatively charged carboxyl or hydroxyl groups that limits attractive forces with the cellular surface. In this paper, we report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, Gd3N@C80(OH)x(NH2)y with a cage surface consisting of positively charged amino groups. This new nanoparticle was characterized by X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), reflection-absorption IR spectroscopy (RAIRS), NMR, and exhibit excellent 1H relaxivity. Previous studies have clearly demonstrated that the cytokine, interleukin-13 (IL-13) effectively targets glioblastoma multiforme (GBM) cells which are known to overexpress IL-13 receptors (IL-13Rα) sites. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y)exhibits enhanced targeting of U-251 GBM cell lines. These results support the hypothesis that positively charged nanoparticles provide enhanced cell surface charge attraction for GBM cellular endocytosis.
Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s DiseasePoster
Here we evaluate the ability to detect changes in phosphorylation levels of ERK and CREB following treatment with Aβ using the SH-SY5Y neuroblastoma cell line.READ MORE
Bioluminescent Kinase Profiling Systems For Characterizing Small Molecule Kinase InhibitorsPoster
In order to profile compounds against a broad panel of kinases, in-house profiling requires rigorous kinase assay development.READ MORE