An HTS-Compatible Plate For Highly Miniaturized Cultures Of Primary Human Bronchial Epithelial Cells At Air-Liquid Interface
Poster Jun 29, 2015
Elizabeth Vu1, Eric Sorscher2, Robert Lowery1, Steven Hayes1
An obstacle to use of HBE-ALI models for high throughput screening (HTS) has been the severely limited supply of tissue samples from patients with unusual CFTR mutations. We have developed a plate for highly miniaturized, arrayed HBE-ALI cultures suitable for use in automated drug screening and mechanistic studies (μALI Plate). Cells cultured on the μALI Plate exhibit hallmarks of HBE biology including beating cilia and mucus production, but require less than one tenth the number of cells compared to conventional Transwell filters. Cells grown in this manner can be visualized directly from the apical surface by immunohistochemistry without the requirement to image through a filter support. Primary cells grown on the μALI Plate will be useful for testing therapeutic interventions directed towards patients with uncommon CFTR variants. Studies of ion channel function, cilia beat frequency and airway surface liquid depth are currently underway. Among other things, the μALI technology has the potential to facilitate personalized approaches to CF therapy, including drug development.
Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAsPoster
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
Histone Deacetylase 6 (HDAC6) As A Therapeutic Target in Chronic Lymphocytic LeukemiaPoster
This study investigates the role of histone deacetylase 6 (HDAC6) in chronic lymphocytic leukemia and establishes it as a novel therapeutic target for the treatment of this disease.READ MORE
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
Comments | 0 ADD COMMENT
International Conference on Nanomedicine and Nanotechnology
Aug 20 - Aug 21, 2018