AN INTEGRATED 1H NMR, GC-MS AND HPLC-(ESI/TOF) BASED METABOLOMICS APPROACH TO THE STUDY OF ACUTE EXERCISE IN HUMAN SERUM METABOLOME
Published: November 24, 2010
There is an increasing concern about the different response and effect that acute exercise induces on diabetic people and a novel insight into this effect can be performed using a metabolomic approach. Metabolomic is becoming widely spread used as a new and powerful tool for discerning significant changes at metabolic level. In this study we aimed to perform a non-targeted analysis and to identify metabolic differences arisen after 30 minutes of acute exercise in both young men suffering from Type 1 Diabetes Mellitus (T1DM) and control individuals by using three different analytical platforms. A first overlook using 1H NMR to identify the largest compounds presents in the sample and a second stage using both gas (GC) and liquid (LC) chromatography coupled to mass spectrometry to identify the minority compounds.