An Ultra-High Throughput Approach to High Content Screening in 1536-Well Format
Poster Feb 01, 2007
Yan Wang, Robert L. Davis and Wayne Bowen
Ultra-high throughput screening (uHTS) using Kalypsys’ technology has enabled unprecedented levels of efficiency and economy in primary screening, and has proved especially useful at rapidly profiling screening hits in selectivity and safety assays.
Combining throughput of over 1 million assay wells per day with on-line storage capacity of over 2 million compounds, the Kalypsys® Integrated Screening System provides unmatched screening efficiency.
To effectively utilize whole cell assay formats in primary and secondary screening, image-based high content readers will be required to have multi-channel capability, rapid read times and compatibility with high-density plate formats.
Laser-scanning fluorescence microplate cytometers, such as the Acumen Explorer, offer whole-well, high content analysis of 1,536- well microplates in less than 10 minutes per plate, while collecting data for up to four colours in multiplex protocols.
The Acumen Explorer thus combines the object-recognition capabilities of image-based systems with read speeds similar to that of bulk readers. Here, we demonstrate the powerful integration of an Acumen Explorer with the Kalypsys® Integrated Screening System, with capability to screen > 300,000 wells per day of high content data.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE