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Analysis of Imported Children's Toys for Heavy Metals

Poster   Jun 15, 2014

 
Analysis of Imported Children's Toys for Heavy Metals
 
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Patricia Atkins, Huifang Lang, William Driscoll

 
 

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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Three cases of mixture analysis by NMR spectroscopy are presented with their respective description of workflow solutions using the ACD/Spectrus platform.

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The Role of K13 in Artemisinin Resistance

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Plasmodium falciparum is evolving resistance to Artemisinin Combination Therapy. The gene with the strongest association with resistance is K13. K13 is an ortholog of the well characterized transcriptional regulator Keap1. In this work we transcriptionally characterized a mutant with a transposon inserted in the K13 promoter region which results in dysregulation of K13 at 2 points of the intraerythrocytic cycle of the life-cycle to identify the processes regulated by K13.

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Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2)

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PfABCk2 gene is ligated into pET21a+ vector with His-tag at C-terminus and transformed into BL-21 (DE3) competent cells that are verified through Miniprep and DNA sequencing. Furthermore, this gene construct is utilized to heterologous express this protein with IPTG and afterwards purified the recombinant protein kinase using nickel affinity chromatography as shown on 10% SDS-PAGE with the expected 36 kDa protein band.

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