Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the POLARstar Omega.
Poster Apr 20, 2010
The Oris™ Cell Migration Assays from Platypus offer certain advantages over traditional migration assays. Especially the TriCoated kits enable the researcher to test the effect of different extracellular matrix components like Collagen I or Fibronectin on migration. In this application note we show the real-time monitoring of cell migration using a POLARstar Omega from BMG LABTECH equipped with a CO2 gas vented system.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE