Analysis of Urine SRMs Using Solid-Phase Micro Extraction, Dynamic Headspace, and Liquid Injection with Comprehensive GCxGC High Res TOFMS
Poster May 04, 2017
David E. Alonso, Joseph E. Binkley, and Jonathan D. Byer
Laboratory medicine began thousands of years ago through urine analysis. Urine was a “Divine Fluid and Window to the Body”. During Babylonian, Egyptian, and through Victorian times, urine was the primary diagnostic tool (Uroscopy).
Today urine is still a favored biofluid for medical diagnostic testing (Urinalysis) because large volumes are easily obtained. In addition, urine is relatively free from interfering proteins and lipids, and it tends to “hold” high concentrations of drugs and metabolites over extended periods of time. Modern, routine clinical tests include the determination of specific gravity and measurement of levels of glucose, nitrates, etc.
- Evaluate different methods of sample introduction
- Implement the use of enhanced, comprehensive (GCxGC) chromatography for separation of urine compounds
- Use modern-day, high-resolution time-of-flight mass spectrometry (HRT) and powerful processing software to quickly and confidently identify compounds in urine
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.