Analytical Pipetting of Serum
Poster Apr 03, 2014
John Thomas Bradshaw, PhD, Leah Flumerfelt, Richard H. Curtis, PhD, Rachel Parshley
Pipetting of solutions is a common practice in chemical and biological laboratories. Many core types of chemical analyses are based upon analytical techniques involving accurate delivery of liquid components. A common tool developed to deliver these liquid components is the handheld micropipette, as well as automated versions of the same. Handheld and automated pipettors have become commonplace tools, especially used in many biological and pharmaceutical laboratories. While these tools are familiar to many, their performance differences when pipetting different types of solutions are often overlooked and neglected aspects that relate directly to the accuracy and reproducibility of pipetting performance. For example, it is commonly known that water pipettes differently than serum. This presentation will address the difference in performance of a handheld micropipette when dispensing water versus various types of animal and human serum. These differences are quantifiable and can be accounted for through careful experimentation and attention to physical pipetting details. Details on achieving ideal performance when pipetting serum will also be discussed.
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE