Analytical Quality by Design (AQbD) for Developing a Validated High-Performance Thin Layer Densitometry Method for Estimating Mangiferin in Human Plasma
Poster Apr 16, 2015
Rajneet Kaur Khurana, Atul Jain, OP Katare, Bhupinder Singh
Of late, Analytical Quality by Design (AQbD) has been gaining increased acceptance in the industrial, academic and regulatory circles. Considered as a science and risk-based approach, AQbD provides rational understanding of the critical method parameters (CMPs) affecting the critical analytical attributes (CAAs) of an analytical method. The present work aims at systematic development of a simple, rapid and highly sensitive bioanalytical high-performance thin-layer densitometry method for the analysis of mangiferin. Initially, the quality target method profile (QTMP) was defined and critical analytical attributes (CAAs) were earmarked. Preliminary studies were conducted for selecting the suitable mobile phase mixture, followed by primary and secondary screening studies employing D-optimal design and Plackett-Burman design, respectively for selecting the ideal mobile phase composition and prioritizing the critically influential method parameters on the CAAs. The CAAs chosen included retardation factor (Rf), peak height, capacity factor, theoretical plates and separation number. Response Surface Methodology (RSM) was conducted as per the face centered cubic design (FCCD) for optimizing volume loaded and plate dimension as the critical method parameters (CMPs) selected initially from the screening studies. The mobile phase containing mixture of ethyl acetate: acetic acid: formic acid: water in 7:1:1:1, v/v/v/v ratio was finally selected as the optimized combination owing to apt chromatographic separation for mangiferin at 262 nm with Rf 0.68±0.02, with all other parameters being within the acceptance limits. Method validation studies revealed high linearity for mangiferin in the concentration range of 50–800 ng/band with r2 =0.998 ± 0.005. The developed method showed high accuracy, precision, ruggedness, robustness, specificity, sensitivity, selectivity, recovery, detection limit (12.1 ng/band) and quantification limit (36.6 ng/band). The bioanalytical method for analysis of mangiferin in plasma revealed the presence of well-resolved peaks and high recovery of mangiferin.
C1 Complement mediates human cord blood serum derived APP α-secretase cleavage activity in vitroPoster
Our results indicate that CBS contains proteins that promote α-secretase like enzymatic activity. LC-MS/MS analysis in CBSF and AgBSF revealed the presence of 142 proteins of which C1 subunits and alpha-2-macroglobulin showed significantly greater levels in αCBSF compared with αAgBSF. further study showed,C1 subunits can enhance sAPPα production and Aβ reduction in cell culture conditionREAD MORE
Fingerprinting the Terpene Profiles of Various Cannabis Strains using GC and GCxGC with High Performance TOFMSPoster
The Pegasus BT 4D facilitates fast and confident cannabis product “fingerprinting” through enhanced two-dimensional chromatographic resolution and high performance TOFMS.
Robust compound identification was achieved through spectral similarity searches of large, well-established databases, mass D determinations, and retention index filtering.
High Speed Analysis: Combining Fast GC with Time-of-Flight Mass Spectrometry for Complex Sample Analysis in Under One MinutePoster
The demand for more efficiency and productivity in the laboratory has led to applications using Fast GC-MS methods for routine analysis in a wide range of fields.READ MORE