Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay
Poster Apr 26, 2016
Stefanie Buesch1 , John Langer2 , Sabine Schaepermeier1, Lubna Hussain2, Jeffrey Bergeron3, Volker Vogel1, Jenny Schroeder1
Conventional in vitro assays are based on cells grown on two-dimensional (2D) substrates, which are not representative of a true in vivo cell environment. Three-dimensional (3D) cell culture methods, in contrast, allow cells to grow in structures resembling more the in vivo environment. Cells can develop cell-cell and cell-extracellular matrix (ECM) interactions in 3D.
While 3D cultures more accurately approach the in vivo environment, it might be difficult to analyze cells in 3D. However, this is not always the case. This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay. Cell viability was determined in two different 3D cell culture systems – the RAFT™ 3D Cell Culture System and classical spheroid cultures generated in ultra-low attachment plates.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE