Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay
Poster Apr 26, 2016
Stefanie Buesch1 , John Langer2 , Sabine Schaepermeier1, Lubna Hussain2, Jeffrey Bergeron3, Volker Vogel1, Jenny Schroeder1
Conventional in vitro assays are based on cells grown on two-dimensional (2D) substrates, which are not representative of a true in vivo cell environment. Three-dimensional (3D) cell culture methods, in contrast, allow cells to grow in structures resembling more the in vivo environment. Cells can develop cell-cell and cell-extracellular matrix (ECM) interactions in 3D.
While 3D cultures more accurately approach the in vivo environment, it might be difficult to analyze cells in 3D. However, this is not always the case. This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay. Cell viability was determined in two different 3D cell culture systems – the RAFT™ 3D Cell Culture System and classical spheroid cultures generated in ultra-low attachment plates.
A New Method for Analyzing MSe/All Ions Fragmentation in Xenobiotic Metabolism StudiesPoster
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE
Extracorporeal shockwave therapy accelerates motor axon regeneration despite a phenotypically mismatched environmentPoster
A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.READ MORE
Comments | 0 ADD COMMENT
World Advanced Therapies & Regenerative Medicine Congress
May 16 - May 18, 2018
3rd Annual Bioprocessing of Advanced Cellular Therapies
May 29 - May 30, 2018
Next Gen Regenerative Medicine & Tissue Engineering
May 29 - May 30, 2018