Apoptosis Inducing Novel microRNA for Breast cancer and Hepatocellular Carcinoma
Poster Apr 23, 2008
Arutselvan Natarajan, Sally J. DeNardo, Mark A. Zern, Senthil K. Venugopal
MicroRNAs (miRNAs) are one of the most prevalent small (~22 nucleotide [nt]) regulatory RNA classes in animals. These miRNAs constitute nearly 1 percent of genes in human genome, making miRNA genes one of the more abundant types of regulatory molecules. MiRNAs have been shown to play important roles in cell development, apoptosis, and other fundamental biological processes. MiRNAs exert their influence through complementary base-pairing with specific target mRNAs, leading in turn to degradation or translational repression of the targeted mRNA. Cancer stem cells (CSC) live indefinitely and become seed for new tumors.
They are not easily killed by most current therapies. We have identified and tested a novel microRNA (miR-491) and demonstrated increased apoptosis in hepatocellular carcinoma (HCC) and in breast cancer cells in vitro. We prepared fluorescent labeled miR-491 by covalent orstreptavidin/biotin coupling to test the apoptosis ability in cancer cells specifically for targeted therapy. The targeting and apoptosis inducing ability was also tested by microscopy using streptavidin gold nanoparticles conjugation. Further studies are ongoing. The preliminary results and current development could be possible to provide a new class of molecules for imaging and therapy of both HCC and breast cancer.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
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