Apoptosis Inducing Novel microRNA for Breast cancer and Hepatocellular Carcinoma
Poster Apr 23, 2008
Arutselvan Natarajan, Sally J. DeNardo, Mark A. Zern, Senthil K. Venugopal
MicroRNAs (miRNAs) are one of the most prevalent small (~22 nucleotide [nt]) regulatory RNA classes in animals. These miRNAs constitute nearly 1 percent of genes in human genome, making miRNA genes one of the more abundant types of regulatory molecules. MiRNAs have been shown to play important roles in cell development, apoptosis, and other fundamental biological processes. MiRNAs exert their influence through complementary base-pairing with specific target mRNAs, leading in turn to degradation or translational repression of the targeted mRNA. Cancer stem cells (CSC) live indefinitely and become seed for new tumors.
They are not easily killed by most current therapies. We have identified and tested a novel microRNA (miR-491) and demonstrated increased apoptosis in hepatocellular carcinoma (HCC) and in breast cancer cells in vitro. We prepared fluorescent labeled miR-491 by covalent orstreptavidin/biotin coupling to test the apoptosis ability in cancer cells specifically for targeted therapy. The targeting and apoptosis inducing ability was also tested by microscopy using streptavidin gold nanoparticles conjugation. Further studies are ongoing. The preliminary results and current development could be possible to provide a new class of molecules for imaging and therapy of both HCC and breast cancer.
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
An Emerging Phenotype of Partial RAG 1/2 Deficiency Among Young Children with Autoimmunity and Viral InfectionsPoster
We describe the natural history of a cohort of 12 patients with confirmed partial RAG1/2 mutations and autoimmunity at a young age. We were seeking the link between viral infections and autoimmunity and tested candidate biomarkers that may reflect the underlying RAG1/2 protein deficiency.READ MORE
Complete alignment identification of CRISPR-Cas9 genomic off-targets using Edit-R CRISPR specificity tool and a comprehensive analysis of positional mismatch tolerancePoster
A web tool that performs complete crRNA specificity checking is introduced. In addition, we evaluated positional off-targeting of the CRISPR system.READ MORE