APPLICATION OF LIQUID CHROMATOGRAPHY WITH UV DETECTION FOR DETERMINATION OF PLANT GROWTH REGULATOR IN COMMON PLANT FERTILIZERS
Poster Aug 25, 2014
Borowczyk K., Wyszczelska-Rokiel M., Stachniuk J., Chwatko G., Glowacki R.
A simple and rapid HPLC-based method was developed for the determination of plant growth regulator in plant fertilizer samples. Plant hormones including indole-3-butyric acid (IBA) are structurally diverse compounds that play an important role in a variety of processes related to plant growth and development. As a minor component of the metabolome, phytohormones play a particular role in the regulation of cell division, enlargement and differentiation, organ formation, seed dormancy and germination [1, 2, 3]. In most cases phytohormones exist in plants either by endogenous secretion or exogenous treatment to achieve various enhanced agriculture characteristics during some critical growth stages. Besides, plant hormones could stimulate the human body or at least affect their immune cells . Therefore, it is necessary to develop a simple and effective methods to detect phytohormones in plant fertilizer samples.
The separation was carried out on Zorbax SB C18 column (4.6 x 150 mm, 5 μm), using acetonitrile/water containing 0.2% acetic acid, pH=2.65 (60:40, v/v) as the isocratic mobile phase at the flow-rate of 1.0 ml/min and column temperature 25 °C. The indole-3-butyric acid was determined with UV detection at λ = 280 nm. The analyte was eluted within 2.7 min. Validation experiments showed that the optimized method has good linearity within the range 1 – 50 nmol/ml, R2 = 0.9993 and high recovery (96.4 % - 114.4 %). The detection limit based on a signal-to-noise ratio was 0.15 nmol/ml. The results indicate that the novel method has advantages of convenience, good sensitivity, high efficiency, and can be useful for the determination of indole-3-butyric acid in common plant fertilizer samples (Clonex, Korzonek Z, Korzonek S).
Profiling Personal Air Pollutant Exposures Using a Wearable Non-Selective Passive WristbandPoster
Characterizing cumulative exposure to air pollutant mixtures is a critical step in understanding disease development. The Fresh Air wristband was tested as a personal exposure assessment tool with promising results.READ MORE
Sport Doping Screening in Biological Matrices by Multi-Dimensional LC-QToFPoster
This work evaluated the performance of 2D LC variant using a QToF setup instead of a triple quadrupole mass spectrometer for the analysis of drug of abuse in urine targeting low and sub ppb level.READ MORE
Analysis of Doping and Forensic Drugs in Urine Using High-Resolution GC/Q-TOFPoster
In this study, we are examining the potential for high resolution accurate mass 7250 GC/Q-TOF equipped with low energy EI source, for both quantitative and screening aspects of doping control and forensic drugs applications.READ MORE