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Assessing the Quality and Quantity of Cell-Free DNA

Assessing the Quality and Quantity of Cell-Free DNA content piece image

For circulating cell free DNA (ccfDNA) to be used
in cancer research successfully, workflow standardization is essential. The new
ISO standard for isolation of ccfDNA from plasma focuses on preanalytical
factors such as preventing cellular DNA release, by using blood stabilization
tubes and dedicated isolation procedures. Accurate determination of ccfDNA is
necessary to ensure good quality and quantity for downstream analysis using
next-generation sequencing or polymerase chain reaction techniques.

Download this poster:

  • For a comparison of ccfDNA quantification and
    qualification methods 

  • To learn how to yield smaller ccfDNA fragments

  • For tips on how to achieve optimal workflow