Assessing the Quality and Quantity of Cell-Free DNA
Poster Feb 19, 2021
For circulating cell free DNA (ccfDNA) to be used
in cancer research successfully, workflow standardization is essential. The new
ISO standard for isolation of ccfDNA from plasma focuses on preanalytical
factors such as preventing cellular DNA release, by using blood stabilization
tubes and dedicated isolation procedures. Accurate determination of ccfDNA is
necessary to ensure good quality and quantity for downstream analysis using
next-generation sequencing or polymerase chain reaction techniques.
Download this poster:
- For a comparison of ccfDNA quantification and
- To learn how to yield smaller ccfDNA fragments
- For tips on how to achieve optimal workflow
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.