ASSESSMENT OF A MICROPATTERNED HEPATOCYTE CO-CULTURE SYSTEM TO DETECT COMPOUNDS THAT CAUSE DRUG INDUCED LIVER INJURY IN HUMANS
Poster Mar 13, 2012
Salman Khetani, Chitra Kanchagar, Stacy Krzyzewski, Michael D. Aleo and Yvonne Will
In vitro approaches which reliably predict in vivo human drug metabolite profiles are highly desired as a means to streamline drug development timelines and substantially reduce development costs. Major metabolites of many compounds can be predicted using traditional in vitro systems such as liver microsomes. The rates of success, however, are typically in the range of 50%. Thus, there is considerable room for improving in vitro systems for predicting human metabolite profiles. This case study demonstrates the increased success of Hepregen’s HepatoPac™ system in identifying primary and secondary circulating and excretory metabolites when compared to liver microsomes, S-9 fractions and primary human hepatocyte suspensions for a series of 27 compounds with known in vivo human metabolite profiles.
LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction for the Analysis of Polar and Non-polar Metabolites in Biological SamplesPoster
Automated robotic sample preparation with hyphenated dual LC-SWATH/MS analysis for lipids and polar metabolites.
Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity AssayPoster
Here we describe a novel 3D NK cell mediated cytotoxicity (CMC) assay.READ MORE
Human iPSC-derived cardiomyocytes: A translational model to predict drug-induced cardiac arrhythmias and long-term toxicityPoster
The results shown here imply that iPSC-derived Cor.4U human cardioymocytes are a translational in vitro cell model for the prediction of clinically relevant drug-induced cardiac arrhythmias and long-term toxicity.READ MORE